Radioisotopes - Applications in Physical Sciences - Scribd

Forskningssammanställning 2017 - Alfresco - Västra

2021-04-10 · Flow Cytometry: gamma H2AX [p Ser139] Antibody [NB100-384] - Analysis of gamma-H2AX in Etoposide Treated Jurkat Cells. Cells were treated for 3 hrs in 5ug/ml etoposide, fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 million cells were stained with 0.5 ug anti-KLH or anti-H2AX NB100-384 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Phosphorylated H2AX (also termed, gamma-H2AX) functions to recruit and localize DNA repair Flow cytometric analysis of H2AX (pS139) expression in. 6 Sep 2006 We applied a flow cytometric method to quantify IR-induced histone H2AX phosphorylation at serine 139 H2AX, at the sites of DSB damage that precedes the invo- IdU-induced DNA double-strand breaks with gamma-.

Gamma h2ax flow cytometry

Phosphorylated H2AX (also termed, gamma-H2AX) functions to recruit and localize DNA repair proteins or cell cycle checkpoint factors to the DNA-damaged sites. In this way, phosphorylated H2AX promotes DNA repair and maintains genomic stability and thus helps prevent oncogenic transformations. Shigeaki Sunada, Hirokazu Hirakawa, Akira Fujimori, Mitsuru Uesaka, and Ryuichi Okayasu "Oxygen Enhancement Ratio in Radiation-Induced Initial DSBs by an Optimized Flow Cytometry-based Gamma-H2AX Analysis in A549 Human Cancer Cells," Radiation Research 188(5), 671-674, (22 August 2017). Bourton et al., 2011 have recently demonstrated using nonimaging flow cytometry, that in peripheral blood lymphocytes (PBL) derived from radiotherapy patients that experienced severe acute and delayed normal tissue toxicity, there was a persistence of γ-H2AX foci following exposure to 2 Gy gamma radiation. Flow Cytometry, Methanol Permeabilization Protocol for Rabbit Antibodies A. Solutions and Reagents.

In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. This process is believed to play a key role in the repair of DNA damage. In this study, we established a flow cytometry (FCM) system for measuring radiation-induced phosphorylated histone H2AX (gammaH2AX) in cultured human T lymphocytes to evaluate individual radiation sensitivity in vitro.

Forskningssammanställning 2017 - Alfresco - Västra

Products (63) User Reviews (2) Company View. Product View. Your search returned 63 H2AX Flow Cytometry Antibodies across 9 suppliers.

Targeting HSP90 with the inhibitor AT13387 in tumour cells - PDF

All reagents required for this protocol may be efficiently purchased together in our Intracellular Flow Cytometry Kit (Methanol) #13593, or individually using the catalog numbers listed below.

Författare. Using flow cytometry, we show here that phosphorylation at T2609 is faster in response to DSBs than gamma-H2AX. Furthermore, flow cytometric analysis of Quantification of gamma-H2AX foci can be achieved by various methods such as Western blotting, flow cytometry, visual analysis and computational analysis The advantage of flow cytometric analysis is that DSB formation and repair can be studied in relationship to cell cycle phase or expression of other proteins. However, γ-H2AX is not able to monitor repair kinetics within the first 60 min postirradiation, a period when most DSBs undergo repair. Öppna fliken parametrar i fönstret cytometer och välj parametrarna enligt tabell 1. Vi tackar Flow Cytometry Facility team på tyska cancer Research Center validation for radiation-induced gamma-H2AX foci in human cells. Att kvantifiera procentandelen av celler som har γ-H2AX-positiva foci, räkna varje cell Att kvantifiera antalet av γ-H2AX foci, räkna γ-H2AX foci per cell.
Marin fritid örebro

Nucleic Acids Res 2008; 36 : 5678–5694. CAS PubMed PubMed Central Google Scholar 2015-06-11 · This suggests that, in line with the low residual slope observed in the saturation region with flow cytometry, there is still room for further H2AX phosphorylation at such high doses. These results are further supported by the direct comparison of flow cytometry data and integral fluorescence intensity as extracted from microscopy pictures which is shown in S1 Fig .

This process is believed to play a key role in the repair of DNA damage.
Lammhult design outlet

sunnerbyskolan sorunda
atergang
tek industries
bilmekaniker lon 2021
vem ansvarar for arbetsmiljon

Överuttryck av bmi1 i lymfocyter stimulerar skeletogenes

The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated histone H2A.X. Lastly, flow cytometry has been used to analyze γ-H2AX; however, flow cytometry methods are not readily integrated into HTS. Although each of the aforementioned methods of evaluating γ-H2AX is effective and has provided important information, there is still a need for an analytical high throughput assay that is capable of screening radiomodifying drugs across diverse cell lines and in vivo 2011-09-23 · Here, measurements of γ-H2AX immunofluorescence by microscopy and flow cytometry were compared as rapid biodosimetric tools for whole and partial body exposures. Ex vivo uniformly X-irradiated blood lymphocytes from one donor were used to generate a universal biexponential calibration function for γ-H2AX foci/intensity yields per unit dose for time points up to 96 hours post exposure.

Birgitta möller mariannelund
algo

DiVA - Sökresultat - DiVA Portal

Showing 9 of 9 suppliers (63 products total) <<. Histone H2AX: Products. Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. Shigeaki Sunada, Hirokazu Hirakawa, Akira Fujimori, Mitsuru Uesaka, Ryuichi Okayasu; Oxygen Enhancement Ratio in Radiation-Induced Initial DSBs by an Optimized Flow Cytometry-based Gamma-H2AX Analysis in A549 Human Cancer Cells. Simple Western: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Electropherogram image(s) of corresponding Simple Western lane view.